| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stupar, Aliyah G (10)
|
VASEVA |
48.16 |
46.70 |
-1.46 |
| 2 |
Fary, Brenna E (10)
|
VAECAT |
50.76 |
49.45 |
-1.31 |
| 3 |
Campbell, Amelia D (10)
|
VASEVA |
49.49 |
50.21 |
0.72 |
| 4 |
Rodrigues, Gabriella A (10)
|
VASEVA |
53.34 |
50.53 |
-2.81 |
| 5 |
Landon, Emma M (10)
|
VAECAT |
|
51.68 |
- |
| 6 |
Shaltayeva, Victoria (10)
|
VASEVA |
57.35 |
53.68 |
-3.67 |
| 7 |
Wojcik, Melissa A (10)
|
VASEVA |
58.88 |
58.61 |
-0.27 |
| 8 |
Banks, Hannah M (10)
|
VASEVA |
1:02.04 |
1:01.08 |
-0.96 |
| 9 |
Graves, Bethany H (10)
|
VASEVA |
|
1:01.74 |
- |
| 10 |
Kohler, Olivia A (10)
|
VATAC |
57.95 |
1:01.77 |
3.82 |
| 11 |
Aquino, Meagan E (10)
|
VAECAT |
1:04.16 |
1:01.85 |
-2.31 |
| 12 |
Townsend, Leah C (10)
|
VAWAC |
1:00.67 |
1:02.09 |
1.42 |
| 13 |
Phillips, Chloe E (10)
|
VAWAC |
59.25 |
1:03.31 |
4.06 |
|
Hoover Smith, Taelyn R (10)
|
VAWAC |
|
DQ |
- |